Sequence Trimming
Automated DNA sequencers occasionally produce poor quality reads, particularly near the sequencing primer site, and toward the end of longer sequence runs. The sequences of clones from DNA libraries frequently contain vector sequence, polyA tails, or other unrelated sequence. Introns and primer sequence frequently flank the sequence of amplified exons. Unless removed by trimming, any of these artifacts will distort your sequence assembly and downstream sequence analysis.
- Compare gene variants to a reference sequence
- Confirm vector constructs
- Assemble viral and bacterial genomes