Casional need for DNA sequencing?
The 4300 DNA analyzer includes basic sequencing software that is operated from a standard Internet browser. The basic sequencing software is designed to meet the needs of users who have an occasional need for DNA sequencing. For moderate to high sequencing demands, or just greater ease of use, LI-COR's e-Seq software gives you a higher level of automation, strong editing tools, and automated reporting that exports files to standard databases or submits files for BLAST searches.
The 4300 DNA analyzer includes basic sequencing software that is operated from a standard Internet browser. The basic sequencing software is designed to meet the needs of users who have an occasional need for DNA sequencing. For moderate to high sequencing demands, or just greater ease of use, LI-COR's e-Seq software gives you a higher level of automation, strong editing tools, and automated reporting that exports files to standard databases or submits files for BLAST searches.
Theoretically yes. We recommend to use the average efficiency of all samples for each gene (the same primer set) to do the quantification. In most cases, only if you want to know how much do tissue, or if treatment specific inhibitory and activatory effects of PCR reaction for the same gene exist in your experiment, you might want use the averaged efficiency for replicates instead for each gene to do the quantification computation. Even though, we recommend you use more replicates